RBS

Part:BBa_K2963011

Designed by: Experiment group advisor of JNU-China   Group: iGEM19_JNU-China   (2019-09-10)


RBS20 - a designed SD sequence for racE

The RBS is a ribosomal binding site in bacterial messenger RNA, generally located around 8 bases upstream of the start codon AUG.The RNA sequence helps recruit the ribosome to the messenger RNA (mRNA) to initiate protein synthesis. Once recruited, tRNA may add amino acids in sequence as dictated by the codons, moving downstream from the translational start site. But different RBS have a important effect on expression of genes. In our project, we design RBS18 for a better expression of racE(racemase enzyme).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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